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Isolation and Antioxidant Mechanism of Polyphenols from Sanghuangporous vaninii

Summary

Researchers found that a medicinal mushroom called Sanghuangporous vaninii grown on mulberry sawdust in China contains exceptionally high levels of polyphenols—powerful antioxidant compounds. They isolated and purified a particularly potent polyphenol mixture called HNMS3, which contains 33 different compounds. Through advanced molecular analysis, they discovered that HNMS3 works by activating eight key proteins in the body to fight oxidative stress, making it potentially beneficial for brain health and preventing nerve degeneration.

Background

Sanghuangporous vaninii is an edible and medicinal macrofungus rich in polyphenols with antioxidant activities. However, polyphenol content and bioactivity vary significantly depending on cultivation substrates used in different regions. This variation has created a need to identify optimal sources and clarify antioxidant mechanisms.

Objective

This study aimed to isolate and characterize polyphenolic components from S. vaninii fruiting bodies cultivated on different substrates and elucidate their antioxidant mechanisms. The research compared five groups of S. vaninii from different cultivation sources to identify the highest polyphenol content and bioactivity.

Results

HNMS from mulberry sawdust in Haining City showed the highest polyphenol content (16.09%) and excellent antioxidant activity. HNMS3, a purified polyphenolic fraction, comprised 33 compounds with strong DPPH and ABTS radical scavenging activities. Network pharmacology identified 257 oxidative stress-related targets and eight hub genes (PPARG, IL-6, STAT3, PTGS2, SRC, MTOR, ESR1, EGFR) regulated by key compounds hispidin, inoscavin A, inoscavin C, and phellibaumin B.

Conclusion

HNMS3 demonstrates excellent antioxidant capacity through multi-target and multi-pathway mechanisms. The identified polyphenolic component shows particular relevance for mental health and neurodegenerative diseases. This research provides molecular insights into S. vaninii antioxidant mechanisms and supports its development as a functional food or medicinal ingredient.
  • Published in:Antioxidants (Basel),
  • Study Type:Experimental Research,
  • Source: PMID: 39765816, DOI: 10.3390/antiox13121487
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