Investigation of Lignocellulolytic Enzymes During Different Growth Phases of Ganoderma lucidum Strain G0119 Using Genomic, Transcriptomic and Secretomic Analyses
- Author: mycolabadmin
- 2018-05-31
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Summary
This research investigated how the medicinal mushroom Ganoderma lucidum breaks down plant material during its growth cycle. The study revealed the complex system of enzymes the fungus uses to digest woody materials and how these enzymes change during different growth stages. This knowledge is important for improving mushroom cultivation.
Impacts on everyday life:
– Helps improve cultivation methods for medicinal mushrooms used in health supplements
– Advances understanding of natural decomposition processes in forests
– Could lead to more efficient production of mushroom-based medicines and supplements
– May contribute to development of eco-friendly ways to break down plant waste
– Could help reduce production costs of beneficial mushroom products
Background
Ganoderma lucidum is a medicinal mushroom known for enhancing human health, with highly profitable products. The fungus degrades plant biomass through secreted enzymes. CAZy proteins are more abundant in its genome compared to other white rot fungi models. However, changes in lignocellulolytic enzymes during growth phases have not been well studied.
Objective
This study aimed to investigate the lignocellulolytic enzymes of G. lucidum strain G0119 to determine which degradative enzymes contribute to its growth through genomic, transcriptomic and secretomic analyses.
Results
The study found that overall cellulase expression was higher than hemicellulase and lignin-modifying enzymes, particularly during fruiting body development. Cellulase and hemicellulase abundances and activities increased after fruiting bodies matured. Lignin-modifying enzymes showed highest expression when mycelia fully spread in the compost. Type I cellobiohydrolase was the most abundant extracellular lignocellulolytic enzyme. AA2 family haem peroxidases were dominant during mycelial growth, while several laccases played roles during primordium formation.
Conclusion
The study provided detailed insights into how G. lucidum’s lignocellulose degradation ability changes during growth phases. The findings revealed key enzymes and their expression patterns during different developmental stages, which could help develop new approaches to accelerate G. lucidum cultivation.
- Published in:PLoS One,
- Study Type:Laboratory Research,
- Source: 10.1371/journal.pone.0198404