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Influence of Temperature on the Bacterial Community in Substrate and Extracellular Enzyme Activity of Auricularia cornea

Summary

This research examined how temperature affects the growth of the edible mushroom Auricularia cornea and the beneficial bacteria in its growing medium. The findings help optimize mushroom farming conditions for better yields and quality. Key impacts on everyday life include: • Improved cultivation methods for this nutritious and medicinal mushroom • More efficient commercial mushroom production through better temperature control • Enhanced understanding of how to maintain beneficial bacterial communities in agricultural systems • Potential applications for optimizing other agricultural fermentation processes • Better quality control methods for mushroom cultivation

Background

Auricularia cornea is a common edible fungus with medicinal value in China. The fruiting bodies contain many nutrients including protein, crude fiber, carbohydrates, amino acids, and microelements. Its polysaccharides have shown blood fat reducing, antioxidant, immune enhancing and anti-cancer properties. Temperature greatly influences A. cornea during growth and can affect polysaccharide and microelement content in the sporocarp.

Objective

To explore the influence of different temperatures (20°C, 25°C, 30°C, 35°C, and 40°C) and culture periods (10, 20 and 30 days) on the activity of A. cornea-associated extracellular enzymes, the structure of bacterial communities in the substrate, and the putative function of dominant bacteria to facilitate commercial cultivation.

Results

Temperature and culture days significantly affected the activities of the four enzymes, with changes not synchronized across conditions. A total of 438,458 high-quality sequences were obtained and clustered into 915 OTUs. The dominant bacterial phyla included Firmicutes (91.6%), Proteobacteria (5.1%), Bacteroidetes (1.6%), and Actionbacteria (1.2%). Bacterial community composition and structure changed notably above 35°C. The membrane transport pathway showed highest average abundance (16.16%) among predicted functional pathways.

Conclusion

Different temperatures significantly impacted both the physiological activity of A. cornea and the bacterial community structure in its substrate. Temperature affected hyphal morphology, extracellular enzyme activities, and bacterial community composition. The findings provide valuable insights for optimizing commercial cultivation conditions for A. cornea.
  • Published in:Mycobiology,
  • Study Type:Experimental Study,
  • Source: 10.1080/12298093.2018.1497795
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