In Vitro Antioxidant and Antimicrobial Activities of Two Edible Mushroom Mycelia Obtained in the Presence of Different Nitrogen Sources

Summary

This research explored how different growing conditions affect the health-beneficial properties of two edible mushroom species. The scientists found that changing the nitrogen source in the growing medium could enhance the mushrooms’ ability to fight microbes and act as antioxidants. This has important implications for developing natural food supplements and functional foods. Impacts on everyday life: • Provides new ways to produce healthier mushroom-based food products • Offers natural alternatives to synthetic preservatives and antioxidants • Helps improve the nutritional value of mushroom-based foods • Contributes to the development of natural antimicrobial ingredients for food preservation

Background

Edible mushrooms are known to have potential antioxidant and antimicrobial properties that can be valuable for nutraceutical and functional food applications. The growth conditions and nitrogen sources used during mycelial cultivation may affect these bioactive properties.

Objective

To evaluate the antioxidant and antimicrobial properties of mycelium ethanolic extracts from two edible mushrooms (Pleurotus ostreatus PBS281009 and Coprinus comatus M8102) grown with different nitrogen sources, and assess their potential as functional food ingredients.

Results

Significant variations were observed in phenol and β-carotene concentrations depending on the nitrogen source used. For P. ostreatus PBS281009, corn extract proved to be the optimal nitrogen source, resulting in the lowest EC₅₀ and MIC values. For C. comatus M8102, peptone was found to be the most effective nitrogen source for enhancing bioactive properties.

Conclusion

The study demonstrated that both mushroom species can produce mycelia with significant antioxidant and antimicrobial properties, with effectiveness varying based on nitrogen source. The findings support the potential use of these mushroom mycelia as ingredients in nutraceuticals and functional food products.
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