In Vitro Antifungal Activity of Olive (Olea europaea) Leaf Extracts Loaded in Chitosan Nanoparticles

Summary

This research explored using olive leaf extracts encapsulated in tiny biodegradable particles to fight harmful fungi that damage crops. The study found that packaging the natural antifungal compounds from olive leaves into nanoparticles made them more effective at stopping fungal growth compared to using the extracts directly. This has important real-world implications: • Could reduce reliance on synthetic chemical fungicides that can harm human health • Provides a natural and environmentally-friendly way to protect crops from fungal diseases • Demonstrates a way to make plant-based medicines more potent and effective • Could lead to safer food production with fewer chemical residues • Shows promise for developing new sustainable agricultural practices

Background

Olive leaf extract contains high levels of phenols and flavonoids like oleuropein and luteolin that have antimicrobial properties. Nanoparticle formulations using natural polymers like chitosan have gained interest for delivering active compounds due to their biodegradability and biocompatibility. There is a need for natural antifungal alternatives to synthetic fungicides that can harm human health and the environment.

Objective

To evaluate the antifungal activity of two different olive leaf extracts (EF1 and EF2) against Fusarium proliferatum, both in free form and encapsulated in chitosan nanoparticles. The study aimed to identify the most appropriate concentration of olive leaf extracts to develop a safe, stable and efficient drug delivery system.

Results

The major compound in both extracts was oleuropein (336 mg/g in EF1 and 603 mg/g in EF2). The nanoparticles showed sizes between 250-270 nm with good stability. At the highest concentration tested (12X), the EF1-loaded chitosan nanoparticles showed the greatest antifungal activity, with 87.96% inhibition of spore germination and 58.13% inhibition of fungal growth. This was more effective than the free extracts or commercial oleuropein.

Conclusion

The olive leaf extract EF1 encapsulated in chitosan nanoparticles demonstrated strong antifungal activity against F. proliferatum, performing better than free extracts. This nanoformulation shows promise as a natural alternative to synthetic fungicides for controlling plant diseases while potentially reducing harmful effects on human health.
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