In vitro and in vivo inhibitory effects and transcriptional reactions of graphene oxide on Verticillium dahliae

Summary

Graphene oxide, a nanomaterial derived from graphene, effectively inhibits the growth of Verticillium dahliae, a fungus that causes devastating wilt disease in cotton and many other plants. The study shows that graphene oxide damages the fungal cell membrane and disrupts key metabolic processes, preventing the fungus from growing and infecting plants. When applied to cotton plants, graphene oxide treatment significantly reduced wilt disease symptoms, suggesting it could be a promising alternative to chemical fungicides for controlling this important agricultural disease.

Background

Verticillium dahliae is a devastating soil-borne fungal pathogen that causes wilt disease in over 200 host species, including cotton. While graphene oxide (GO) has shown antimicrobial properties against bacteria and some fungi, research on its antifungal effects against plant pathogenic fungi remains limited.

Objective

This study aimed to investigate the inhibitory effects of graphene oxide on V. dahliae growth and spore germination, and to elucidate the underlying molecular mechanisms through transcriptome analysis.

Results

GO significantly inhibited mycelial growth and spore germination in a dose-dependent manner. Transcriptome analysis revealed 1,149 differentially expressed genes during hyphal elongation and 1,078 during spore germination. Downregulated genes primarily encoded membrane proteins, oxidoreductases, and transporters, indicating disrupted cell membrane integrity and compromised cellular functions.

Conclusion

GO suppresses V. dahliae proliferation by disrupting cell membrane integrity and impairing metabolic pathways. GO treatment reduced cotton wilt symptoms in pot experiments, suggesting potential for developing GO-based antifungal agents for managing Verticillium wilt disease.
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