Improved Mycelia and Polysaccharide Production of Grifola frondosa by Controlling Morphology with Microparticle Talc

Summary

This research discovered that adding microscopic talc particles during mushroom fermentation can improve the production of beneficial compounds called polysaccharides from the medicinal mushroom Grifola frondosa. The talc particles help control how the mushroom grows, leading to better yields of these valuable molecules. Impacts on everyday life: – More efficient production of natural medicinal compounds from mushrooms – Potential for more affordable mushroom-based supplements and medicines – Improved manufacturing processes for fungal-derived products – Advances in sustainable biotechnology methods – Better understanding of how to optimize mushroom growth for commercial production

Background

Mushroom polysaccharides are biological macromolecules with immunomodulatory, antitumor, anti-inflammatory, hypoglycemic, and hepatoprotective activities. Mushroom mycelia showed pellet, clump and/or filamentous morphologies during fermentation which affect metabolite production. Microparticle-enhanced cultivation using materials like Talc has been proposed as a morphology engineering strategy to control fungal growth and improve productivity.

Objective

To investigate the effect of microparticle Talc addition on mycelial morphology, fermentation performance, monosaccharide compositions of polysaccharides and enzymes activities associated with polysaccharide synthesis in Grifola frondosa, and find relationships between polysaccharide biosynthesis and morphological changes.

Results

Addition of Talc (3.0-6.0 g/L) decreased pellet diameter and increased S-fraction mycelia percentage. Maximum mycelial biomass of 19.25 g/L and exo-polysaccharide of 3.12 g/L were achieved at 6.0 g/L Talc, while highest mycelial polysaccharide yield of 0.24 g/g occurred at 3.0 g/L Talc. Talc significantly altered monosaccharide compositions in polysaccharides, with highest mannose percentage (62.76%) and lowest glucose percentage (15.22%) corresponding to changes in polysaccharide synthesis enzyme activities.

Conclusion

Addition of Talc microparticles effectively controlled G. frondosa morphology and improved mycelia and polysaccharide production by affecting morphological appearance and polysaccharide-biosynthesis related enzyme activities. The optimal Talc concentration was 3.0-6.0 g/L for enhanced fermentation performance.
Scroll to Top