Identification of Degenerate Nuclei and Development of a SCAR Marker for Flammulina velutipes
- Author: mycolabadmin
- 2014-09-15
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Summary
This research investigated genetic abnormalities in the commercially important enoki mushroom (Flammulina velutipes) that cause poor growth and reduced mushroom production. Scientists identified specific genetic markers that can detect problematic strains and discovered that these issues are linked to changes in a particular gene region. This finding has important implications for mushroom farming.
Impacts on everyday life:
– Helps mushroom farmers identify and avoid using poor quality strains
– Contributes to more reliable mushroom production and better crop quality
– May lead to improved breeding methods for commercial mushroom varieties
– Could help reduce economic losses in mushroom farming operations
– Supports sustainable production of nutritious food crops
Background
Flammulina velutipes is a major edible mushroom that has shown abnormalities affecting crop production, including slow vegetative growth, compact mycelial mat formation, and reduced or absent fruiting bodies. These abnormalities can lead to economic losses, but their underlying causes are not fully understood.
Objective
To characterize and identify degenerate nuclei in F. velutipes strains and develop a specific molecular marker to differentiate between wild-type and degenerate strains. The study aimed to investigate the morphologies and fruiting capabilities of monokaryons from wild-type and degenerate strains through test crossing.
Results
Only one monokaryotic group of the degenerate strains and its hybrid strains showed abnormal phenotypes. A sequence-characterized amplified region (SCAR) marker linked to the degenerate monokaryon was identified. Comparative analyses revealed a degenerate-specific genomic region in a telomere containing a putative helicase gene, which was transferred from one nuclear type to another.
Conclusion
The study successfully identified that only one aberrant nucleus of the two nuclei in the degenerate strain was responsible for degeneracy. A specific molecular marker was developed to detect degenerate strains. The findings suggest that the transfer of a genomic region containing a putative helicase gene may be responsible for the observed abnormalities in F. velutipes.
- Published in:PLOS One,
- Study Type:Laboratory Research,
- Source: 10.1371/journal.pone.0107207