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Identification of a Key Protein Set Involved in Moniliophthora perniciosa Necrotrophic Mycelium and Basidiocarp Development

Summary

This research analyzed proteins involved in the development of a fungus that causes witches’ broom disease in cacao trees. The study identified key proteins that control how the fungus grows and produces reproductive structures. This knowledge could help develop better ways to control this devastating plant disease. Impacts on everyday life: • Could lead to better protection of cacao crops and chocolate production • May help reduce economic losses in cacao-producing regions • Contributes to understanding fungal diseases that affect food security • Could lead to new fungal control strategies in agriculture • Helps protect the livelihoods of cacao farmers

Background

Moniliophthora perniciosa is a hemibiotrophic fungus causing witches’ broom disease in cacao. The fungus has two distinct phases – a biotrophic phase with monokaryotic mycelium that initiates disease, and a necrotrophic phase with dikaryotic mycelium leading to tissue necrosis. Understanding the necrotrophic phase and basidiocarp formation is crucial for disease control.

Objective

To conduct the first proteomic analysis of M. perniciosa developmental phases when cultivated on bran-based solid medium, identifying key proteins involved in mycelium and basidiocarp development.

Results

259 proteins were identified and categorized into nine functional classes. An 82-key protein set was identified as highly relevant to fungal development, with 30 proteins considered crucial for specific developmental stages. Key proteins were related to energy metabolism, protein folding, morphogenesis, and were potentially involved in primordium and basidiocarp formation.

Conclusion

This first proteomic study of M. perniciosa developmental stages identified crucial proteins involved in necrotrophic mycelium and basidiocarp development. The identified proteins may represent useful candidates for further analysis related to fungal spread and pathogenesis.
  • Published in:Fungal Genetics and Biology,
  • Study Type:Laboratory Research,
  • Source: 10.1016/j.fgb.2021.103635
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