Identification and Evaluation of Reference Genes for qRT-PCR Studies in Lentinula edodes

This research focused on improving the accuracy of genetic studies in shiitake mushrooms by identifying the most reliable reference genes for measuring gene expression. This is important because shiitake mushrooms have significant nutritional and medicinal properties that researchers are trying to understand better at the molecular level. Impact on everyday life: – Helps develop more accurate methods for studying beneficial compounds in mushrooms – Contributes to improving cultivation and quality of edible mushrooms – Supports development of better medicinal compounds from mushrooms – Advances our understanding of how mushrooms produce their health-beneficial substances