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Hydrogen Sulfide Maintains Mitochondrial Homeostasis and Regulates Ganoderic Acids Biosynthesis by SQR Under Heat Stress in Ganoderma lucidum

Summary

This research reveals how hydrogen sulfide (H2S) helps mushrooms cope with heat stress by protecting their cellular powerhouses (mitochondria). The findings show that H2S acts as a natural protective molecule that helps maintain normal cellular energy production when fungi are exposed to high temperatures. This discovery has important implications for understanding how organisms adapt to environmental stress. Impacts on everyday life: • Helps improve cultivation methods for medicinal mushrooms • Advances our understanding of how organisms cope with climate stress • Could lead to better methods for preserving food fungi • May inspire new approaches for protecting cells from heat damage • Provides insights for developing stress-resistant crops

Background

Hydrogen sulfide (H2S) has recently been recognized as an important gaseous transmitter with multiple physiological effects across various species. Previous studies showed H2S alleviated heat-induced ganoderic acids (GAs) biosynthesis, an important quality index of Ganoderma lucidum. However, a comprehensive understanding of H2S’s physiological effects and molecular mechanisms in G. lucidum remained unexplored.

Objective

To investigate the role of hydrogen sulfide in maintaining mitochondrial homeostasis and regulating ganoderic acids biosynthesis under heat stress conditions in Ganoderma lucidum, and to elucidate the underlying molecular mechanisms.

Results

Heat treatment reduced mitochondrial membrane potential and DNA copy number in G. lucidum. Increasing intracellular H2S levels improved MMP, mtDNAcn, oxygen consumption rate and ATP content under heat stress. H2S was found to activate sulfide-quinone oxidoreductase (SQR) and complex III, maintaining mitochondrial homeostasis. SQR mediated the negative regulation of H2S on ganoderic acids biosynthesis under heat stress. The study also found that SQR could be persulfidated under heat stress conditions.

Conclusion

The study reveals a novel physiological function of H2S signaling in G. lucidum under heat stress, where it maintains mitochondrial homeostasis through the SQR-Com III pathway and regulates ganoderic acids biosynthesis. This finding has broad implications for understanding environmental response mechanisms in microorganisms.
  • Published in:Redox Biology,
  • Study Type:Laboratory Research,
  • Source: 10.1016/j.redox.2024.103227
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