Honeybee associated Aspergillus niger AW17 as a source of selective anticancer compounds with cytotoxicity evaluation in human cancer cell lines

Summary

Scientists discovered that a fungus found in honeybees can produce compounds that effectively kill cancer cells while leaving healthy cells largely unharmed. The fungus (Aspergillus niger AW17) contains unique bioactive compounds including high levels of oleic acid and a rare compound called pachymic acid. When tested against four types of human cancer cells, the fungal extract was particularly effective against liver and colorectal cancer. This finding suggests that exploring specialized environments like beehives could lead to new cancer treatments with fewer side effects.

Background

Cancer remains a leading global health challenge requiring novel therapeutic agents. Natural products, particularly from fungi, have historically provided approximately 60% of approved anticancer drugs. The honeybee microbiome represents an understudied ecological niche potentially harboring microorganisms with unique metabolic capabilities.

Objective

To investigate the anticancer potential of Aspergillus niger strain AW17 isolated from honeybees through chemical characterization and systematic evaluation of cytotoxicity across multiple human cancer cell lines.

Results

The extract demonstrated selective cytotoxicity with IC50 values of 5.22 µg/ml (HepG2), 26.78 µg/ml (Caco-2), 34.18 µg/ml (A549), and 55.91 µg/ml (MCF-7), while sparing normal cells (WI-38: 1454.7 µg/ml, HFB-4: 668.3 µg/ml). Chemical analysis revealed oleic acid (28.88%), pachymic acid (18.93%), and other unique compounds. Different cancer cell lines exhibited distinct death mechanisms including apoptosis, necrosis, and cell cycle arrest.

Conclusion

Honeybee-associated Aspergillus niger AW17 produces selective anticancer compounds with remarkable selectivity for cancer cells over normal cells, suggesting potential for targeted cancer therapy development and highlighting the value of exploring specialized ecological niches in natural product discovery.
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