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High-quality genome assembly and annotation of Porodaedalea mongolica and Porodaedalea schrenkiana provide insights into potential industrial and medical application

Summary

Scientists sequenced the genomes of two medicinal wood-decay fungi species (Porodaedalea mongolica and P. schrenkiana) for the first time using advanced sequencing technology. These fungi produce beneficial compounds with anti-inflammatory and anticancer properties, and can degrade environmental pollutants. The detailed genetic information revealed how these fungi break down wood and create bioactive compounds, opening new possibilities for medical treatments and industrial applications like environmental cleanup.

Background

Porodaedalea species are wood-inhabiting fungi in the order Hymenochaetales, widely distributed in temperate regions of the Northern Hemisphere. These fungi are known for their saprophytic, pathogenic, and medicinal properties. However, genomic studies on Porodaedalea remain extremely limited despite their potential applications.

Objective

This study employed third-generation sequencing technology to obtain high-quality genomes of Porodaedalea mongolica and Porodaedalea schrenkiana using an optimized assembly strategy for highly heterozygous genomes. The goal was to explore lignocellulose decomposition strategies and identify potential biological functions and evolutionary patterns.

Results

P. mongolica genome was assembled into 46.26 Mb with 55 contigs and 96% BUSCO completeness; P. schrenkiana into 48.65 Mb with 66 contigs and 96.1% completeness. Both species showed distinct lignocellulose degradation strategies with different secreted CAZyme profiles. The genomes revealed potential for environmental pollutant degradation and secondary metabolite production including terpenoids and polysaccharides.

Conclusion

High-quality genomes of P. mongolica and P. schrenkiana provide valuable foundation for understanding Porodaedalea evolution and lignocellulose degradation. These genomic resources demonstrate potential for industrial applications in bioremediation and medical applications through bioactive metabolite production. The study establishes an assembly strategy applicable to other highly heterozygous fungal genomes.
  • Published in:G3 (Bethesda),
  • Study Type:Genomic Analysis,
  • Source: 10.1093/g3journal/jkaf195, PMID: 40996866
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