H3K4ME2 ChIP-Seq Reveals Epigenetic Landscape During Mushroom Formation and Novel Developmental Regulators of Schizophyllum commune
- Author: mycolabadmin
- 2021-04-14
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Summary
This research reveals how chemical modifications to DNA-packaging proteins help control mushroom development in fungi. Using advanced molecular techniques, scientists mapped these modifications and identified key genes that control mushroom formation. Impact on everyday life:
– Improved understanding of mushroom cultivation for food production
– Better methods for controlling fungal growth in agricultural settings
– Potential applications in biotechnology and medicine through understanding of fungal development
– Enhanced ability to produce beneficial compounds from mushrooms
– New tools for studying gene regulation in other organisms
Background
Mushroom formation represents the most complex multicellular development in fungi. While comparative genomics and transcriptomics have provided insights into mushroom development regulation in the model species Schizophyllum commune, little is known about epigenetic regulation during this process.
Objective
To map the distribution of H3K4me2 histone modification during monokaryotic and dikaryotic development in S. commune using ChIP-Seq, and identify novel developmental regulators through analysis of differential epigenetic marks.
Results
The study identified 6032 and 5889 H3K4me2 sites during monokaryotic and dikaryotic development respectively, strongly enriched near translation initiation sites. While the overall epigenetic landscape was similar between conditions, 837 sites showed differential enrichment, associated with 965 genes. Six transcription factor genes were enriched during dikaryotic development. Deletion of two of these genes (fst1 and zfc7) resulted in arrested mushroom development at the primordia stage.
Conclusion
H3K4me2 ChIP-Seq proved to be a powerful tool for mapping epigenetic landscape changes during mushroom development and identifying novel developmental regulators. This is the first report of ChIP-Seq use in a mushroom-forming basidiomycete, providing new insights into epigenetic regulation during mushroom formation.
- Published in:Scientific Reports,
- Study Type:Laboratory Research,
- Source: 10.1038/s41598-021-87635-8