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GlPP2C1 Silencing Increases the Content of Ganoderma lingzhi Polysaccharide (GL-PS) and Enhances SLT2 Phosphorylation

Summary

This research discovered how to increase the production of beneficial polysaccharides in the medicinal mushroom Ganoderma lingzhi by manipulating specific genes. These polysaccharides have important health benefits and are used in medicine and functional foods. The study found that by silencing a particular gene (GlPP2C1), they could boost the mushroom’s production of these beneficial compounds. Impacts on everyday life: • Could lead to more efficient production of medicinal mushroom supplements • May help create more potent natural medicines from mushrooms • Could reduce the cost of mushroom-based health products • Provides new ways to enhance the therapeutic properties of medicinal mushrooms • Contributes to the development of better natural alternatives to synthetic drugs

Background

Polysaccharides have attracted significant attention in the food industry due to their diverse biological activities. Research on polysaccharide synthesis mechanisms has mainly focused on crucial enzymes in the synthesis pathway, while other regulatory genes remain unexplored. Ganoderma lingzhi polysaccharides (GL-PS) have important medicinal properties including antioxidant, antibacterial, immune regulation, antitumor, and liver protection functions.

Objective

To investigate the role of the GlPP2C1 gene, which encodes a phosphoprotein type 2C phosphatase, in regulating polysaccharide synthesis in Ganoderma lingzhi and explore its relationship with SLT2 phosphorylation.

Results

GlPP2C1 silencing increased IPS content by 25%, EPS content by 33%, and β-1,3-D-glucan content by 36% compared to wild-type strains. The PP2C-silenced strains showed enhanced cell wall resistance and increased SLT2 phosphorylation levels. The study demonstrated that GlPP2C1 silencing increased polysaccharide biosynthesis through the SLT2-mediated pathway.

Conclusion

The study revealed for the first time that protein phosphatase GlPP2C1 plays a negative regulatory role in GL-PS biosynthesis and cell wall stress resistance in Ganoderma lingzhi, while SLT2 acts as a positive regulator. GlPP2C1 silencing increases polysaccharide content through enhanced SLT2 phosphorylation, providing new insights into polysaccharide biosynthesis regulation in medicinal mushrooms.
  • Published in:Journal of Fungi,
  • Study Type:Laboratory Research,
  • Source: 10.3390/jof8090949
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