Genomic and Transcriptomic Approaches Provide a Predictive Framework for Sesquiterpenes Biosynthesis in Desarmillaria tabescens CPCC 401429

Summary

This research explores how a specific mushroom species produces valuable chemical compounds called sesquiterpenes through analyzing its genetic makeup. The study helps us understand how fungi naturally make molecules that could be useful for medicine and industry. Impacts on everyday life: – Provides new ways to produce natural compounds for medicines and fragrances – Helps develop more efficient methods for manufacturing pharmaceutical ingredients – Advances our understanding of how to harness fungi for producing useful chemicals – Could lead to discovery of new anti-cancer compounds – Contributes to more sustainable production methods for valuable chemical compounds

Background

Terpenoids are structurally diverse secondary metabolites with wide applications in pharmaceutical, fragrance and flavor industries. Desarmillaria tabescens CPCC 401429 is a basidiomycetous mushroom that produces anti-tumor melleolides. No previous studies have thoroughly investigated the sesquiterpenes biosynthetic potential in Desarmillaria or related genus.

Objective

This study aimed to unravel the phylogeny, terpenome, and functional characterization of unique sesquiterpene biosynthetic genes of D. tabescens CPCC 401429 through genomic and transcriptomic analysis.

Results

The genome contained 15,145 protein-encoding genes across 50.36 Mb. Phylogenetic analyses supported reclassification of D. tabescens to the genus Desarmillaria. Genome mining revealed 12 putative sesquiterpene synthases, with 6 belonging to a novel diverse Clade IV. Yeast cells expressing DtSTS9 and DtSTS10 produced diverse sesquiterpene compounds, indicating these enzymes are highly promiscuous producers.

Conclusion

The genomic and transcriptomic analyses provide new insights into the phylogeny, sesquiterpene synthase diversity, and functional significance of Desarmillaria species. The results encourage further research on uncharacterized sesquiterpene synthases of Basidiomycota, their biological functions, and potential applications as sources of secondary metabolites.
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