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Genome-wide identification and transcriptome analysis of the cytochrome P450 genes revealed its potential role in the growth of Flammulina filiformis

Summary

Scientists identified 59 cytochrome P450 genes in the golden needle mushroom (Flammulina filiformis), an economically important edible fungus. These genes appear to control the mushroom’s growth and development, particularly the elongation of the stalk. By understanding how these genes work, researchers can potentially improve mushroom cultivation and develop new varieties with better growth characteristics. This research provides valuable insights into the genetics of mushroom growth and development.

Background

Cytochrome P450 (CYP450) genes play essential roles in metabolism, stress responses, and growth regulation in plants, but their functions in edible fungi remain largely unexplored. Flammulina filiformis is an economically important mushroom that lacks comprehensive analysis of its CYP450 gene family. This study aims to identify and characterize CYP450 genes at the genome-wide level.

Objective

To identify and characterize CYP450 genes in F. filiformis, investigate their expression patterns across different developmental stages, and explore their potential roles in fungal growth and adaptation.

Results

59 CYP450 genes were identified and classified into 6 clades. Gene duplication analysis revealed 3 tandem and 3 segmental repeat pairs. Transcriptomic analysis showed significant enrichment of oxidoreductase activity genes during developmental transitions. 11 CYP450 genes showed increasing expression from lower to upper stipe regions, correlating with stipe elongation.

Conclusion

The study provides a comprehensive foundation for understanding CYP450 gene function in F. filiformis. The identified genes, particularly those with increasing expression in the stipe, may regulate stipe elongation and fruiting body development. These findings have implications for molecular breeding and understanding growth mechanisms in edible fungi.
  • Published in:BMC Genomics,
  • Study Type:Genome-wide Association Study,
  • Source: 10.1186/s12864-025-11555-4, PMC11974101, 40197176
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