Genetic Diversity of the Edible Mushroom Pleurotus sp. by Amplified Fragment Length Polymorphism
- Author: mycolabadmin
- 2012-07-06
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Summary
This research examined the genetic makeup of different strains of oyster mushrooms (Pleurotus species) using DNA fingerprinting techniques. The study helps identify and distinguish between different varieties of these commercially important mushrooms, which is crucial for mushroom cultivation and breeding programs.
Impacts on everyday life:
• Helps ensure quality control in commercial mushroom production
• Supports development of improved mushroom varieties for food production
• Contributes to maintaining genetic diversity in cultivated mushrooms
• Assists farmers in selecting the best mushroom strains for cultivation
• Helps protect consumers by ensuring accurate identification of mushroom varieties
Background
The genus Pleurotus comprises an important group of edible mushrooms with high nutritional value, therapeutic properties, and various environmental and biotechnological applications. These fungi have great commercial value in the global market of cultivated mushrooms, ranking third in annual production. However, cultivated strains can undergo genetic erosion from selective breeding, making it important to assess genetic diversity.
Objective
To evaluate the usefulness of a simplified Amplified Fragment Length Polymorphism (AFLP) technique for studying genomic diversity and identification of Pleurotus sp. strains.
Results
The AFLP analysis generated 371 DNA fragments, including 308 polymorphic bands. Each strain showed highly specific AFLP profiles that allowed unambiguous distinction between the 21 Pleurotus fungi. Genomic similarity coefficients ranged from 0.0 to 0.750, with an average of 0.378. The strains were grouped into two major clusters and one independent lineage.
Conclusion
The AFLP-PstI method proved effective for identification and molecular characterization of Pleurotus sp. strains, demonstrating its potential applicability in distinguishing between different species and strains of this commercially important mushroom genus.
- Published in:Current Microbiology,
- Study Type:Laboratory Research,
- Source: 10.1007/s00284-012-0175-7