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Genetic and Functional Analysis of the Zn(II)2Cys6 Transcription Factor HADA-1 in Hypsizygus marmoreus

Summary

This research investigated a key genetic regulator in edible mushrooms that controls their growth and development. The study found that a specific gene called hada-1 acts as a master switch affecting multiple aspects of mushroom biology, including how they grow and produce mushroom caps. Impact on everyday life: • Improved understanding of mushroom growth could lead to better cultivation methods for edible mushrooms • May help develop more efficient commercial mushroom production techniques • Could contribute to breeding better mushroom varieties for food production • Advances our knowledge of how fungi regulate their growth and development • May lead to improved methods for controlling fungal growth in agricultural settings

Background

Zn(II)2Cys6 transcription factors are known to be critical for reproductive growth and sexual development in fungi, but their specific roles in Basidiomycota remain poorly understood.

Objective

To investigate the function of the hada-1 gene, which encodes a Zn(II)2Cys6 transcription factor, in the basidiomycete mushroom Hypsizygus marmoreus and understand its role in growth, development and metabolism.

Results

In hada-1-silenced strains, researchers observed decreased mycelial growth rates, angulated hyphae, and hindered fruiting body development. The mitochondria showed morphological changes from oval to dumbbell or linear shapes, with decreased complex II, III and V activities and ATP content. Carbohydrate metabolism-related enzyme activities were reduced, and the strains showed higher ROS levels with increased sensitivity to oxidative stress.

Conclusion

The study demonstrates that hada-1 functions as a global regulator in H. marmoreus, playing crucial roles in mycelial growth, fruiting body development, carbon metabolism, mitochondrial stability, and oxidative stress response.
  • Published in:Applied Microbiology and Biotechnology,
  • Study Type:Experimental Research,
  • Source: 10.1007/s00253-021-11175-4
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