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Gene Expression Related to Trehalose Metabolism and its Effect on Volvariella Volvacea Under Low Temperature Stress

Summary

This research investigated how mushrooms deal with cold storage problems by studying a sugar molecule called trehalose. Scientists found that mushroom strains with higher levels of this sugar survived better in cold storage. They also discovered that spraying mushrooms with trehalose solution during growth helped them stay fresh longer in the refrigerator. Impacts on everyday life: – Could lead to better preservation methods for mushrooms in home refrigerators – May help reduce food waste from spoiled mushrooms – Could result in longer shelf life for fresh mushrooms in stores – Might enable more efficient cold chain transportation of mushrooms – Could potentially reduce costs for consumers by extending mushroom storage life

Background

Volvariella volvacea is an edible fungus sensitive to low temperatures. Under conventional 4°C storage, it experiences wilting, effluent and decay phenomena which limits industry growth. Trehalose is an important osmolyte that helps fungi resist adverse conditions, but its role in V. volvacea’s low temperature response is not well understood.

Objective

To investigate the role of trehalose metabolism in V. volvacea’s response to low temperature stress by comparing gene expression and trehalose content between temperature-sensitive (V23) and temperature-tolerant (VH3) strains, and to evaluate if exogenous trehalose application can improve low temperature tolerance.

Results

The trehalose content in VH3 was consistently higher than V23 under low temperature stress. Expression of trehalose-6-phosphate phosphatase (TPP) decreased in both strains initially but remained higher in VH3 during extended exposure. Trehalose phosphorylase (TP) showed increased expression early in cold exposure. Fruit bodies treated with exogenous trehalose showed significantly improved sensory traits during 4°C storage compared to controls.

Conclusion

Trehalose plays an important role in V. volvacea’s response to low temperature stress. Higher trehalose content in VH3 likely contributes to its superior cold tolerance compared to V23. The TP gene appears to be crucial in trehalose synthesis during cold stress. Exogenous trehalose application during cultivation can improve fruit body tolerance to low temperature storage, particularly in temperature-sensitive strains.
  • Published in:Scientific Reports,
  • Study Type:Laboratory Research,
  • Source: 10.1038/s41598-018-29116-z
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