Fungal Strain Matters: Colony Growth and Bioactivity of the European Medicinal Polypores Fomes fomentarius, Fomitopsis pinicola and Piptoporus betulinus

Summary

This research demonstrates that different strains of the same medicinal mushroom species can have very different properties and health benefits. The study focused on three types of bracket fungi traditionally used in European medicine and found that their ability to fight bacteria and other fungi varies significantly depending on which specific strain is used. This has important implications for both traditional medicine and modern drug development. Impacts on everyday life: • Better quality control for medicinal mushroom products through proper strain selection • More effective natural antimicrobial treatments by using optimal fungal strains • Improved understanding of how geographic origin and growing conditions affect medicinal properties • More reliable identification of beneficial mushroom species for foraging and cultivation • Enhanced potential for developing new antibiotics from mushroom compounds

Background

Polypores have been widely used in traditional Chinese medicine and are becoming increasingly popular worldwide due to their various bioactivities including anti-cancer, anti-inflammatory, antiviral and immuno-enhancing effects. While their secondary metabolites have been studied extensively, the importance of fungal strain selection for bioactivity and metabolite production has not been investigated for these Basidiomycetes.

Objective

To screen and compare multiple strains from three medicinal polypore species (Fomes fomentarius, Fomitopsis pinicola and Piptoporus betulinus) regarding their growth rates, optimum growth temperatures, and antimicrobial/antifungal properties of ethanolic fruit body extracts.

Results

Significant strain-specific differences were found in growth rates, optimal temperatures, secondary metabolite production and bioactivities within species. F. pinicola and P. betulinus extracts showed strong antibiotic activities against Bacillus subtilis and Staphylococcus aureus (MICs 31-125 μg/mL). Antifungal activities against Aspergillus flavus, A. fumigatus, Absidia orchidis and Candida krusei were often strain-specific (MICs 125-1000 μg/mL). Phylogenetic analysis revealed distinct lineages within F. fomentarius based on geographic origin and host specificity.

Conclusion

Fungal strain selection is crucial for bioactivity and metabolite production in medicinal polypores. Reliable species identification followed by extensive screening for optimal strains is essential for identifying bioactive material. Substrate and geographic origin influence strain properties and should be considered in strain selection.
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