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Function of Transcription Factors PoMYB12, PoMYB15, and PoMYB20 in Heat Stress and Growth of Pleurotus ostreatus

Summary

This research investigated how certain genes help oyster mushrooms (Pleurotus ostreatus) cope with heat stress and control their growth. The findings show that manipulating specific genes can help mushrooms better survive high temperatures and grow more efficiently. This has important real-world implications: • Could lead to development of heat-resistant mushroom strains for summer cultivation • May help reduce crop losses during hot weather • Could improve year-round mushroom production efficiency • May lead to faster-growing mushroom varieties • Could help make mushroom farming more economically viable in warmer climates

Background

MYB transcription factors (TFs) have been extensively studied in plant abiotic stress responses and growth/development, but their role in heat stress response and development of Pleurotus ostreatus remains unclear. P. ostreatus is among the most widely cultivated edible mushrooms globally, valued for its nutritional and medicinal attributes. During summer cultivation, elevated temperatures can cause mycelial scalding and retard development, making heat stress response mechanisms particularly important to understand.

Objective

To investigate the function of PoMYB12, PoMYB15, and PoMYB20 transcription factors in P. ostreatus by generating mutant strains using RNA interference (RNAi) and overexpression (OE) techniques and studying their roles in heat stress response and growth/development.

Results

The mycelia of OE-PoMYB12, OE-PoMYB20, and RNAi-PoMYB15 mutant strains showed positive effects under heat stress at 32°C, 36°C, and 40°C. These mutant strains promoted growth and development of P. ostreatus compared to wild-type strains, and facilitated recovery of growth after 24h of 36°C heat stress. Expression of PoMYB12 and PoMYB20 supported mycelium’s response to heat stress and enhanced growth, while PoMYB15 produced opposite effects.

Conclusion

The expression of PoMYB12 and PoMYB20 supports mycelial heat stress response and enhances growth and development of P. ostreatus, while PoMYB15 produces opposite effects. These findings provide important reference for breeding and technological improvement of P. ostreatus varieties and deeper understanding of heat stress response and growth regulation in macro-fungi.
  • Published in:International Journal of Molecular Sciences,
  • Study Type:Laboratory Experimental Study,
  • Source: 10.3390/ijms241713559
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