Flammulina velutipes stem regulates oxidative damage and synthesis of yolk precursors in aging laying hens by regulating the liver–blood–ovary axis

Summary

This study shows that adding golden needle mushroom stems to the feed of aging laying hens improves egg production by reducing oxidative damage and boosting reproductive hormones. The mushroom stem, normally a waste product, works through a liver-ovary signaling pathway to enhance the production of yolk-building proteins and improve the quality and number of developing eggs. The treatment was particularly effective at doses of 4-6% supplementation and also had beneficial side effects like reducing abdominal fat and improving eggshell thickness.

Background

Egg production in aging laying hens declines due to decreased liver and ovarian function, reduced antioxidant capacity, and lower reproductive hormone levels. The golden needle mushroom (Flammulina velutipes) exhibits antioxidant, anti-inflammatory, and hypolipidemic properties. This study investigates the therapeutic effects of F. velutipes stem on the liver-blood-ovary axis in aging laying hens.

Objective

To reveal the therapeutic effects of Flammulina velutipes stem on the liver-blood-ovary signaling axis and investigate the underlying mechanisms by which it regulates oxidative stress, reproductive hormones, and yolk precursor synthesis in aging laying hens.

Results

FVS supplementation significantly increased egg production, ovarian development, and antioxidant enzyme levels (GSH-Px, T-SOD, T-AOC) while decreasing malondialdehyde in liver, serum, and ovaries. FVS reduced ovarian apoptosis, increased reproductive hormone levels and their receptors, and enhanced yolk precursor synthesis genes (ApoVLDL II, ApoB, VTG II) through estrogen receptor activation.

Conclusion

Dietary supplementation with Flammulina velutipes stem maintains productive performance in aging laying hens by alleviating oxidative stress and regulating the liver-blood-ovary signaling axis, resulting in improved follicular development and yolk precursor synthesis.
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