Fishing in Fungi: Visualisation of Mushroom Virus X in the Mycelium of Agaricus bisporus by Fluorescence In Situ Hybridisation
- Author: mycolabadmin
- 2020-06-01
- View Source
Summary
This research developed a new way to visualize viruses inside mushroom tissue using fluorescent markers. This technique helps scientists better understand how viruses spread through commercial mushrooms and cause disease. Impact on everyday life:
• Could lead to better disease control in mushroom farming
• May help reduce crop losses in commercial mushroom production
• Could result in higher quality mushrooms for consumers
• Demonstrates potential for similar techniques in other crop diseases
• May lead to improved food security through better plant disease management
Background
Agaricus bisporus is a commercial mushroom crop susceptible to mushroom virus X (MVX), a complex of viruses that causes symptoms like bare patches and mushroom cap discoloration. Limited understanding exists about how these viruses localize and move within the mushroom mycelium.
Objective
To develop a non-destructive fluorescence in situ hybridisation (FISH) method for targeting and visualizing two MVX viruses (AbV6 and AbV16) within A. bisporus mycelium to better understand their distribution patterns and behavior.
Results
The MVX strain associated with bare patch disease showed high AbV6 signal near growing culture edges and around putative vacuoles. The browning disease strain displayed high AbV16 signals in reticulating hyphal networks in a compartmentalized pattern. The two viruses were found in discrete areas of the mycelium with different distribution patterns. FISH detected low virus levels in cultures that tested negative by RT-PCR, suggesting higher sensitivity.
Conclusion
FISH was successfully adapted to detect, localize and characterize distribution patterns of two different mycoviruses within A. bisporus mycelium. The technique proved reproducible, cost-effective and non-destructive, allowing visualization of both high and low virus levels. The observed localization patterns suggest specific virus movement through the mycelium.
- Published in:Journal of Microbiological Methods,
- Study Type:Laboratory Research,
- Source: 10.1016/j.mimet.2020.105913