MYCOLab Ai Logo - gold and army green

Family Identification and Functional Study of Copper Transporter Genes in Pleurotus ostreatus

Summary

This research investigated how copper transporter genes help oyster mushrooms (Pleurotus ostreatus) cope with heat stress. The study found that these genes play a crucial role in protecting mushroom cells from heat damage by maintaining proper copper levels and cell membrane integrity. Impact on everyday life: – Improved understanding of how to grow mushrooms in warmer conditions – Potential development of heat-resistant mushroom strains for agriculture – Better mushroom cultivation techniques for farmers – Enhanced food security through more resilient crop varieties – Possible applications in other heat-sensitive crops

Background

The copper transport (COPT/Ctr) family plays a crucial role in maintaining metal homeostasis in organisms, with many species relying on Ctrs for transmembrane copper uptake. While Ctr family genes have been widely studied in plants, there is limited research on their role in edible mushrooms.

Objective

To identify and characterize copper transporter genes in Pleurotus ostreatus and investigate their role in heat stress tolerance and cell membrane integrity. The study specifically focused on analyzing four PoCtr genes and exploring the biological function of PoCtr4 through transformed strains.

Results

The study identified four PoCtr genes in P. ostreatus. Addition of exogenous copper ions (Cu2+) increased mycelial temperature tolerance and maintained cell membrane integrity. PoCtr4 overexpression enhanced heat stress tolerance and H2O2 resistance. Under mild stress (32°C), OE-PoCtr4 promoted mycelial growth and maintained cell membrane integrity. The transformed strains showed improved recovery after heat stress at 40°C.

Conclusion

The research demonstrated that PoCtr4 plays a significant role in heat stress tolerance in P. ostreatus by maintaining cell membrane integrity and enhancing heat resistance through regulation of Cu2+ homeostasis. The addition of trace amounts of copper can promote mycelial growth and repair cell membrane damage caused by high temperatures.
  • Published in:International Journal of Molecular Sciences,
  • Study Type:Laboratory Research,
  • Source: 10.3390/ijms252212154
Scroll to Top