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Extraction Optimization and Bioactivity of Polysaccharides from Ganoderma leucocontextum Spores

Summary

Researchers developed an improved method to extract beneficial polysaccharides from Ganoderma leucocontextum (white Ganoderma), a rare mushroom from the Tibetan plateau. The extracted compound GLSP-A1 showed strong anti-inflammatory effects by reducing inflammatory markers and reduced signs of aging in test organisms by decreasing harmful reactive oxygen species and cellular aging pigments. This work suggests the mushroom polysaccharide could potentially be used in dietary supplements to help slow aging.

Background

Oxidative stress is associated with aging and age-related diseases. Ganoderma leucocontextum, a rare subspecies found in the Tibetan plateau, possesses potent biological activities including antitumor, hypoglycemic, and immune regulation properties. Natural polysaccharides have attracted attention as antioxidants and anti-aging agents due to their superior biological activity and low toxicity.

Objective

To optimize extraction and purification of polysaccharides from Ganoderma leucocontextum spores using three-phase partitioning (TPP) method and evaluate their anti-inflammatory and anti-aging bioactivity.

Results

Optimized TPP extraction conditions yielded 5.3% polysaccharide with improved extraction efficiency compared to traditional hot water extraction. GLSP-A1 had a molecular weight of 15,750 Da and contained fucose, glucosamine, galactose, glucose, and mannose. GLSP-A1 significantly reduced IL-6 and IL-1β expression in macrophages and decreased ROS levels and lipofuscin accumulation in C. elegans by 49.34% and 33.15% respectively.

Conclusion

TPP provides a straightforward, effective, and environmentally friendly extraction method for polysaccharides from Ganoderma leucocontextum spores. The purified polysaccharide GLSP-A1 demonstrated favorable anti-inflammatory and anti-aging properties, suggesting potential application as a dietary supplement for anti-aging purposes.
  • Published in:Pharmaceuticals (Basel),
  • Study Type:Experimental Study,
  • Source: PMC11859662, PMID: 40006054
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