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Expression Profile of Laccase Gene Family in White-Rot Basidiomycete Lentinula edodes under Different Environmental Stresses

Summary

Researchers studied how shiitake mushrooms control 14 different laccase genes in response to environmental changes like temperature, light, and food sources. Laccases are enzymes that help mushrooms break down wood and other tough plant materials. The study found that different genes activate under different conditions, helping the mushroom adapt and develop fruiting bodies efficiently. This research helps improve mushroom cultivation and understanding of how fungi survive in changing environments.

Background

Laccases are ligninolytic enzymes that play important roles in filamentous fungi, including fruiting-body formation and lignin degradation. The fruiting-body development in Lentinula edodes is complex and significantly affected by environmental conditions. Understanding laccase gene expression under different stresses is critical for efficient mushroom cultivation.

Objective

To analyze the expression profile of 14 laccase genes in L. edodes under different environmental stresses including varying carbon sources, temperatures, and photoperiods. The study aimed to facilitate understanding of laccase function and to identify key genes for further functional research.

Results

All laccase genes were significantly regulated by carbon source variation, with Lelcc5 and Lelcc6 induced by lignosulfonate and most genes upregulated in glucose. Most genes showed decreased expression at 39°C but significant upregulation at 10°C after 24h. Five laccase genes showed similar expression profiles to blue light receptor genes under 12h light/12h dark treatment, suggesting involvement in light adaptation and fruiting-body formation.

Conclusion

The study demonstrated comprehensive expression profiles of 14 laccase genes across different environmental stresses, indicating their involvement in L. edodes adaptation to changing environments. Results suggest functional divergence among laccase genes and provide valuable information for understanding white-rot fungi’s adaptive capacity and laccase engineering research.
  • Published in:Genes (Basel),
  • Study Type:Experimental Research,
  • Source: PMID: 31888265, DOI: 10.3390/genes10121045
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