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Exploring the Wound Healing Potential of Hispidin

Summary

Hispidin, a natural compound from medicinal mushrooms, shows promise as a wound healing treatment. Research demonstrates that hispidin can kill harmful bacteria, stimulate skin cell growth and migration, reduce inflammation, and improve wound closure when applied as an ointment. The 5% hispidin ointment performed as well as or better than standard wound treatments in rat studies, with no toxic side effects observed, suggesting potential for future wound care products.

Background

Hispidin is a polyphenolic compound derived from medicinal mushrooms Phellinus and Inonotus species, known for antioxidant and anti-inflammatory properties. While hispidin shows promise for biomedical applications, its specific wound healing potential remains largely unexplored. This research aims to comprehensively investigate hispidin’s effects on wound healing through combined in vitro and in vivo approaches.

Objective

This study investigates the wound healing effects of hispidin through in vitro scratch assays on NIH-3T3 fibroblasts and in vivo wound models in rats, while evaluating its antimicrobial properties and safety profile. The research assesses hispidin’s potential as a topical wound healing agent compared to standard treatments.

Results

Hispidin demonstrated antimicrobial activity against all tested organisms, particularly effective against Staphylococcus epidermidis (MIC 100 μg/disc). Hispidin enhanced NIH-3T3 cell viability and promoted wound closure with increased FGF21, TGF-β1, EGF, and VEGF levels. In vivo studies showed 5% hispidin ointment improved wound contraction, epithelialization, and tissue regeneration while reducing pro-inflammatory cytokines and increasing antioxidant activities. Acute dermal toxicity studies showed no adverse effects at 2000 mg/kg.

Conclusion

Hispidin demonstrates significant wound healing potential through antimicrobial, anti-inflammatory, antioxidant, and regenerative properties. The 5% w/w hispidin ointment showed comparable or superior efficacy to the standard nitrofurazone treatment across multiple wound healing parameters. These findings support hispidin as a promising candidate for novel wound care applications with an excellent safety profile.
  • Published in:Nutrients,
  • Study Type:In vitro and In vivo Experimental Study,
  • Source: PMID: 39339761
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