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Evaluation of MucorGenius® Mucorales Real-Time PCR Assay with Formalin-Fixed Paraffin-Embedded Tissue Specimens

Summary

Researchers tested a rapid fungal detection test called MucorGenius® on tissue samples from trauma patients with serious fungal infections. The test successfully identified dangerous mold-like fungi in 92% of infected samples with no false positives. This quick test could help doctors diagnose and treat dangerous fungal wound infections faster in trauma patients.

Background

Early identification of mucormycetes in trauma-related invasive fungal wound infections improves patient outcomes. The MucorGenius® assay is a commercially available multiplex real-time PCR that can provide results within 3 hours, making it a promising diagnostic tool.

Objective

To evaluate the performance of the MucorGenius® Mucorales Real-Time PCR assay using formalin-fixed paraffin-embedded tissue specimens from combat-injured patients with fungal infections.

Results

From 31 patients contributing 63 specimens, 70% had Apophysomyces or Saksenaea fungi. The assay detected Mucorales DNA in 58 specimens with 5 false-negative findings, achieving 92% sensitivity (95% CI: 82-97%) and 100% specificity (95% CI: 16-100%). The assay successfully amplified DNA from genera not targeted by the panel.

Conclusion

This first assessment of MucorGenius® with fixed tissue specimens in trauma-related infections demonstrated high sensitivity and specificity compared to sequencing. The assay’s potential for rapid results offers promise for fast diagnosis of invasive fungal infections in trauma settings.
  • Published in:Open Forum Infectious Diseases,
  • Study Type:Diagnostic Evaluation Study,
  • Source: 10.1093/ofid/ofae631.2281
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