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Elucidation of Higher Basidiomycetes Enzyme Activity Based on Mushroom Inoculum Properties

Summary

This research investigated how different properties of mushroom cultures affect their ability to produce important industrial enzymes. Scientists found that factors like the age and form of the mushroom culture significantly impact enzyme production, with different mushroom species responding differently to various growing conditions. This knowledge is valuable for optimizing enzyme production for industrial applications. Impacts on everyday life: • More efficient production of enzymes used in eco-friendly paper manufacturing • Better methods for producing enzymes used in biofuel production • Improved techniques for manufacturing textile processing enzymes • More sustainable production of enzymes used in food processing • Enhanced understanding of how to cultivate medicinal mushrooms efficiently

Background

Understanding how different properties of mushroom inoculum affect enzyme production is important for optimizing lignocellulose-degrading enzyme production in medicinal mushrooms. Previous research has not fully explored how inoculum form, age, size and precultivation medium impact enzyme activity.

Objective

To evaluate how five different mushroom inoculum properties (form, age, size, and precultivation medium) affect the production of lignocellulose-deconstructing enzymes during submerged fermentation of mandarin marc.

Results

Homogenization of submerged mycelium improved enzyme production in C. unicolor 305 and G. lucidum 447. Finely chopped mycelial agar enhanced CMCase and xylanase production in T. multicolor 511 and LiP secretion in C. unicolor 305 and G. lucidum 447. Four-day-old inoculum was optimal for laccase and MnP production in G. lucidum 447 and T. multicolor 511, while 7-day-old mycelium worked best for C. unicolor 305. Twelve-day-old homogenized mycelium resulted in lowest laccase activity but highest cellulase activity.

Conclusion

The study demonstrated that enzyme activity and accumulation profiles vary significantly depending on the mushroom species and inoculum properties. Adapting individual fungi to lignocellulose during maintenance and inoculum cultivation can be beneficial for optimizing production of specific enzymes.
  • Published in:International Journal of Medicinal Mushrooms,
  • Study Type:Laboratory Research,
  • Source: 10.1615/IntJMedMushrooms.2020035655
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