Effects of Resinous Compounds from Pine Trees on Spore Germination and Mycelial Growth of a Nematophagous Fungus, Esteya vermicola
- Author: mycolabadmin
- 4/9/2025
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Summary
Researchers tested whether a beneficial fungus called Esteya vermicola could be injected into pine trees to fight pine wilt disease. However, they discovered that natural resinous compounds found in pine trees strongly inhibit the growth of this fungus. The findings suggest that trunk injection of this fungus may not be an effective treatment because the tree’s own defensive compounds prevent the fungus from establishing itself and fighting the harmful nematodes.
Background
Pine wilt disease caused by pine wood nematodes (PWN) causes severe damage worldwide. Esteya vermicola, a nematophagous fungus, has been explored as a biological control agent against PWN. However, understanding the interaction between this fungus and pine trees remains limited, particularly regarding the effects of pine resinous compounds.
Objective
This study aimed to evaluate the effects of resinous compounds on spore germination and mycelial growth of E. vermicola in vitro to determine the feasibility of using trunk injection of E. vermicola spore suspension for biological control of pine wilt disease.
Results
All 14 resinous compounds showed inhibitory effects on spore germination (36.9-100% SGIR) and mycelial growth (0-100% MGIR) of E. vermicola. Individual compounds (+)α-pinene, (-)α-pinene, and (-)limonene showed 100% inhibition of both spore germination and mycelial growth. The mixture of resinous compounds achieved 100% SGIR and 91.8% MGIR.
Conclusion
Pine resinous compounds strongly inhibit spore germination and mycelial growth of E. vermicola, suggesting that trunk injection of E. vermicola spore suspension may be ineffective for controlling pine wilt disease due to inhibition by naturally occurring pine resins and compounds released during injection.
- Published in:Mycobiology,
- Study Type:Experimental Laboratory Study,
- Source: PMID: 40213411, DOI: 10.1080/12298093.2025.2484866