Effects of Exogenous Ascorbic Acid on the Mycelia Growth and Primordia Formation of Pleurotus Ostreatus

Summary

This research investigated how vitamin C (ascorbic acid) affects the growth and development of oyster mushrooms. Scientists found that adding vitamin C helped the mushrooms grow faster and start forming their initial fruiting bodies earlier than normal. This was achieved through changes in gene activity and protein production in the mushroom cells. Impacts on everyday life: • Could lead to more efficient commercial mushroom cultivation methods • May reduce production time for edible mushrooms • Demonstrates potential for using natural compounds to enhance mushroom growth • Could help lower costs for mushroom producers and consumers • Provides insights for developing better mushroom growing techniques

Background

Primordia formation is the first and most critical step in the development of fruiting bodies of edible fungi. Understanding factors that influence this process is important for mushroom cultivation.

Objective

To investigate the effects of exogenous ascorbic acid (ASA) on Pleurotus ostreatus mycelia growth and primordia formation, including analysis of protein content, enzyme activity, and gene expression.

Results

The addition of exogenous ASA accelerated the growth rate of P. ostreatus mycelia and advanced primordia formation timing. Protein content in mycelia showed an initial decrease followed by an increase with rising ASA concentrations. Within a certain concentration range, ASA significantly enhanced AAO activity. The expression levels of development regulating genes and blue light receptor coding genes were altered following ASA addition.

Conclusion

Exogenous ascorbic acid influences P. ostreatus development through changes in gene expression, particularly affecting light morphogenesis genes PoWC-1 and PoWC-2, which in turn impacts development regulating genes Pofst3 and Pofst4, leading to earlier primordia formation.
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