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Effects of Atmospheric and Room Temperature Plasma (ARTP) Mutagenesis on Physicochemical Characteristics and Immune Activity in vitro of Hericium erinaceus Polysaccharides

Summary

This research demonstrates how scientists can improve the medicinal properties of lion’s mane mushroom using a special mutation technique called ARTP. The mutated mushroom strain produced more beneficial compounds called polysaccharides that can boost immune system function. Impact on everyday life: – Potential for more effective natural supplements and medicines derived from mushrooms – More efficient production of medicinal mushrooms for health products – Better understanding of how to enhance beneficial compounds in edible fungi – Possibility of developing improved strains of other medicinal mushrooms – Advancement in natural approaches to immune system support

Background

Hericium erinaceus is an edible and medicinal mushroom commonly consumed in China and other oriental countries, known for its gastrotherapeutic and other beneficial health effects. Polysaccharides isolated from its fruiting bodies are considered major bioactive compounds with various pharmacological activities including immuno-modulating, anti-tumor, antioxidant, and gastro-protective properties.

Objective

To investigate the differences in physicochemical characteristics and immune activity between polysaccharides isolated from fruiting bodies cultivated by ARTP mutagenic strains (321) and the original strain (0605) of H. erinaceus, and to assess the effect of ARTP mutagenesis on polysaccharide immunostimulant activities.

Results

The ARTP mutant strain showed 22% higher fruiting body yield and 16% higher polysaccharide content compared to the original strain. Significant differences were found in molecular weight distribution and monosaccharide compositions between mutant and original strains. The NO, IL-6, IL-10, IL-1β, and TNF-α production by macrophages was enhanced by 20% ethanol precipitated polysaccharides from the ARTP mutant strain compared to the original strain.

Conclusion

ARTP mutagenesis effectively improved both yield and polysaccharide content in H. erinaceus while altering the structural characteristics and enhancing the immunological activities of the polysaccharides. This provides a theoretical reference for applying ARTP mutagenesis in quality breeding of H. erinaceus and lays foundation for better exploitation of H. erinaceus resources.
  • Published in:Molecules,
  • Study Type:Laboratory Research,
  • Source: 10.3390/molecules24020262
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