Early Development of Moniliophthora perniciosa Basidiomata and Developmentally Regulated Genes
- Author: mycolabadmin
- 2009-08-04
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Summary
This research examined how a devastating fungal pathogen that affects cocoa trees develops its reproductive structures and which genes control this process. The study combined detailed microscopic observations with genetic analysis to understand how the fungus transitions from simple thread-like growth to forming complex mushroom-like structures that spread the disease.
Impacts on everyday life:
• Helps develop better methods to control cocoa tree diseases that affect chocolate production and farmer livelihoods
• Advances understanding of how fungi develop and reproduce, which is important for both plant diseases and beneficial fungi
• Provides new genetic targets that could be used to prevent the spread of agricultural plant diseases
• Contributes to protecting cocoa farming communities’ economic stability in affected regions
• Demonstrates how combining visual and genetic analysis helps understand complex biological processes
Background
Moniliophthora perniciosa is a hemibiotrophic fungus that causes Witches’ Broom Disease in cocoa trees. The pathogen’s life cycle concludes with basidiocarp production in dead host tissues, generating millions of basidiospores that reinfect young tissues. Understanding the mechanisms of the sexual phase could help develop control strategies for the disease.
Objective
To analyze the morphological changes in mycelium prior to basidiomata emergence and identify genes involved in basidiomata development through expression analysis of selected EST genes from a non-normalized cDNA library representative of M. perniciosa fruiting stage.
Results
Morphological analysis revealed stages of hyphal nodules, aggregation, initial primordium and differentiated primordium. Macroarray analysis identified up-regulated genes in primordial phases including hydrophobin coding, glucose transporter, Rho-GEF, Rheb, extensin precursor and cytochrome p450 monooxygenase genes. Down-regulated genes included calmodulin, lanosterol 14 alpha demethylase and PIM1. RT-qPCR analysis showed distinct expression patterns for aegerolysin genes in primordia and basidiomata stages. Glucose transporter gene expression increased after stress, coinciding with decreased adenylate cyclase gene transcription.
Conclusion
The study provides the first comparative morphologic analysis of early M. perniciosa basidiomata development both in vivo and in vitro, along with the first description of genes expressed during this fungal life cycle stage. The identification of genes with increased expression during fruiting opens new possibilities for controlling fungal spread and studying biological processes leading to basidiomycete fruiting.
- Published in:BMC Microbiology,
- Study Type:Laboratory Research,
- Source: 10.1186/1471-2180-9-158