Dynamic Succession of Substrate-Associated Bacterial Composition and Function During Ganoderma lucidum Growth
- Author: mycolabadmin
- 2018-06-13
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Summary
This research examined how bacterial communities in growing material change during the cultivation of the medicinal mushroom Ganoderma lucidum. The study found that bacterial populations shift significantly as the mushroom grows, particularly during its elongation stage. Understanding these changes could help improve commercial cultivation of this valuable medicinal fungus.
Impacts on everyday life:
• Better cultivation methods for medicinal mushrooms could make them more available and affordable
• Improved understanding of microbial interactions could lead to more sustainable agricultural practices
• More efficient production of natural health products containing beneficial compounds
• Enhanced knowledge of how microorganisms work together in agricultural systems
• Potential for developing new approaches to growing other valuable mushroom species
Background
Ganoderma lucidum is a valuable medicinal fungus widely distributed in China that grows alongside a complex microbial ecosystem in its substrate. The fruiting bodies and spores are highly appreciated as health products due to their richness in polysaccharides and triterpenoids, which strengthen the immune system and inhibit tumor formation. While studies have characterized changes in G. lucidum’s body during growth, changes in the microbial community of the surrounding substrate have been poorly studied.
Objective
To analyze the bacterial community dynamics in the substrate during four typical growth stages of G. lucidum using next-generation sequencing and reveal the composition and functions of substrate-associated bacterial communities.
Results
A total of 598,771 sequences from 12 samples were obtained and assigned to 22 bacterial phyla. Proteobacteria and Firmicutes were the dominant phyla. Bacterial community composition and diversity significantly differed between the elongation stage and the other three growth stages. LEfSe analysis revealed many bacterial taxa with significantly higher abundance at the elongation stage. Functional pathway prediction showed significant changes between growth stages, with decreased membrane transport and increased metabolism-related pathways at elongation stage.
Conclusion
The growth of G. lucidum significantly affected substrate physicochemical properties and bacterial community composition. Changes in substrate properties likely drove bacterial community changes. The diversity, structure and predicted functions of the bacterial community differed most at elongation stage. Decreased nutrients and moisture indicated vigorous growth at elongation stage, suggesting nutrient supply is crucial at this stage for both G. lucidum and substrate bacteria. The findings provide insight into G. lucidum-bacteria-substrate relationships that could benefit industrial cultivation.
- Published in:PeerJ,
- Study Type:Experimental Research,
- Source: 10.7717/peerj.4975