Draft Genome Sequence of Lactarius deliciosus Strain CBS 582.63 from the CBS-KNAW Culture Collection

Summary

Scientists have successfully mapped the genetic blueprint (genome) of the saffron milk cap mushroom, an edible mushroom commonly found in the Northern Hemisphere. This research provides the first detailed look at the genes of this interesting fungus, which is unique because it can produce natural rubber similar to some plants. The study revealed that this mushroom has about 12,753 genes in its DNA. Impacts on everyday life: • Helps understand how mushrooms produce natural rubber, potentially leading to new sustainable rubber sources • Advances our knowledge of edible mushroom genetics, which could improve cultivation methods • Could lead to better understanding of mushroom properties for food and medicinal applications • May help in developing more efficient farming techniques for this commercially valuable mushroom • Contributes to conservation efforts by better understanding this species’ biology

Background

Lactarius mushrooms, also known as milk cap mushrooms, are commonly found in the Northern Hemisphere and produce natural rubber (high-molecular-weight cis-polyisoprene). Lactarius deliciosus, known as the saffron milk cap mushroom, belongs to the family Russulaceae in the Basidiomycota subdivision of Fungi.

Objective

To sequence, assemble and annotate the genome of Lactarius deliciosus strain CBS 582.63.

Results

The sequencing generated approximately 3 Gb of data with 61× coverage and a mean read length of 7,965 bp from 386,212 total reads. The assembled genome has a total length of 54 Mbp with 841 contigs, an N50 value of 116,430 bp, and a GC content of 51.98%. Annotation identified 12,753 genes.

Conclusion

The complete draft genome sequence of Lactarius deliciosus strain CBS 582.63 was successfully sequenced and annotated, providing valuable genomic resources for future studies of this economically important mushroom species.
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