Development of High Cordycepin-Producing Cordyceps militaris Strains

Summary

This research focused on developing improved strains of the medicinal mushroom Cordyceps militaris that can produce higher amounts of cordycepin, a valuable compound with multiple health benefits. Instead of using genetic modification, the researchers used natural breeding methods to create new strains. The study successfully produced a new strain that makes 35% more cordycepin than existing strains. Impacts on everyday life: • Could lead to more affordable and effective natural health supplements • Provides a sustainable way to produce medicinal compounds without synthetic chemicals • Demonstrates how traditional breeding methods can improve natural medicine production • Could help make beneficial fungal-derived medicines more widely available • Shows potential for developing other improved medicinal mushroom strains

Background

Cordyceps militaris, known as Dong-Chong-Xia-Cao, is an entomopathogenic fungus that produces cordycepin, a valuable bioactive compound with various therapeutic properties. C. militaris can be artificially cultured and produces the highest cordycepin content among Cordyceps species, making it widely used as an herb or functional food in East Asia.

Objective

To develop new strains of C. militaris that produce higher cordycepin content than parent strains through mating-based sexual reproduction, without using mutation techniques.

Results

Eight out of 35 mating combinations successfully formed stroma with normal perithecia. The mated strain KSP8 produced the highest cordycepin content (6.63 mg/g) among all tested strains, showing a 35% increase compared to the best parent strain KACC44461 (4.89 mg/g). The silkworm pupae medium yielded the highest cordycepin production across all strains tested.

Conclusion

Through natural mating-based sexual reproduction, new C. militaris strains with increased cordycepin production were successfully developed. The study demonstrates that mating can produce strains with higher cordycepin content than parent strains without requiring mutation techniques, which has potential applications in health food and medicine industries.
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