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De Novo Sequencing and Transcriptome Analysis of Pleurotus eryngii subsp. tuoliensis (Bailinggu) Mycelia in Response to Cold Stimulation

Summary

This research examined how an edible mushroom called Bailinggu responds to cold temperatures at the genetic level. The study revealed the complex ways that the mushroom’s cells adapt to cold conditions, which is essential for successful mushroom cultivation. Impact on everyday life: – Helps improve commercial mushroom production methods – Contributes to developing more efficient cultivation techniques – Could lead to reduced energy costs in mushroom farming – Advances understanding of how organisms adapt to cold conditions – May help develop cold-resistant crop varieties

Background

Pleurotus eryngii subsp. tuoliensis (Bailinggu) is a valuable edible mushroom commercially cultivated in China since 1997 and popular in Asian markets. Cold stimulation of mature mycelia is crucial for triggering primordia initiation and successful fruiting body production. However, the molecular mechanisms involved in mycelia response to cold stimulation remain unclear.

Objective

To perform comparative transcriptomic analysis using RNA-Seq technology to better understand gene expression regulation during different temporal stages of cold stimulation in Bailinggu mycelia. The specific objectives were to identify relevant functional gene groups and signaling pathways associated with cold stimulation and screen/identify EST-SSR markers for genetic diversity and mapping studies.

Results

A total of 21,558 Bailinggu mycelia unigenes were assembled and annotated from four libraries. GO and KEGG analysis revealed differentially expressed genes associated with cell wall/membrane stabilization, calcium signaling, MAPK pathways, and metabolism. Key genes involved in cold response included those for hydrophobins, desaturases, heat shock proteins, and antioxidant enzymes. 607 potential EST-SSR loci were identified, with 92% polymorphism rate in validation tests using wild strains.

Conclusion

The study revealed complex regulatory networks triggered during cold stimulation, involving genes for signal transduction, cell growth, and metabolite biosynthesis. The identified cold-responsive genes and pathways provide new insights into molecular mechanisms of cold adaptation in Bailinggu. The validated SSR markers will be valuable for genetic studies and breeding programs.
  • Published in:Molecules,
  • Study Type:Transcriptome Analysis,
  • Source: 10.3390/molecules21050560
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