Curing Two Predominant Viruses Occurring in Lentinula edodes by Chemotherapy and Mycelial Fragmentation Methods
- Author: mycolabadmin
- 2022-02-01
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Summary
This research focused on finding ways to eliminate harmful viruses from shiitake mushrooms, which are important edible fungi in commercial cultivation. The study tested two methods to remove viruses: using an antiviral drug and breaking up the mushroom’s thread-like cells. The findings show that breaking up cells was more effective at eliminating both viruses, while the antiviral drug only worked on one virus type. Mushrooms freed from these viruses grew better and looked healthier.
Impacts on everyday life:
• Improved methods for producing healthier shiitake mushrooms for consumption
• Potential for better mushroom yields in commercial farming
• More efficient cultivation techniques for mushroom growers
• Possibility of lower production costs leading to more affordable mushrooms
• Enhanced quality control in mushroom cultivation
Background
Lentinula edodes mycovirus HKB (LeV-HKB) and L. edodes partitivirus 1 (LePV1) are major mycoviruses identified in L. edodes germplasm. These viruses can affect the growth and development of shiitake mushrooms, but methods for eliminating them had not been fully evaluated.
Objective
To evaluate and compare the effectiveness of two different methods – ribavirin treatment and mycelial fragmentation – for eliminating LeV-HKB and LePV1 viruses from Lentinula edodes.
Results
Mycelial fragmentation successfully eliminated both LeV-HKB and LePV1 viruses. Ribavirin treatment was only effective in eliminating LeV-HKB but did reduce LePV1 concentration in the eighth successive subcultures compared to untreated controls. Virus-cured strains demonstrated faster mycelial growth rates and higher colony density than infected strains. LeV-HKB infection was found to affect pigmentation in both plate- and bag-cultivated mycelia of L. edodes strain L135.
Conclusion
The study demonstrated that mycelial fragmentation is more effective than ribavirin treatment for virus elimination in L. edodes, as it can eliminate both LeV-HKB and LePV1. The removal of these viruses resulted in improved growth characteristics, suggesting their negative impact on mushroom cultivation.
- Published in:Journal of Virological Methods,
- Study Type:Experimental Research,
- Source: 10.1016/j.jviromet.2021.114370