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Culture Conditions for Production of Biomass, Adenosine, and Cordycepin from Cordyceps sinensis CS1197: Optimization by Desirability Function Method

Summary

This research focused on developing efficient methods to cultivate the medicinal fungus Cordyceps sinensis in laboratory conditions, as natural populations are threatened by overharvesting. The researchers successfully optimized growing conditions to produce the fungus and its beneficial compounds adenosine and cordycepin. Impacts on everyday life: • Provides a sustainable source of this valuable medicinal fungus without harming natural populations • Makes beneficial compounds more accessible for potential medical treatments • Demonstrates how traditional medicines can be produced through modern biotechnology • Could help reduce the cost of Cordyceps-based medicines and supplements • Supports conservation of wild Cordyceps populations in their natural habitat

Background

Cordyceps sinensis (CS) is a valuable entamophagous fungus used in traditional Chinese medicine as a natural tonic to strengthen vitality and promote longevity. It parasitizes caterpillar stage of ghost moths and produces a fruiting body used as an herbal remedy. Due to overharvesting threatening its natural populations, there is a need to develop efficient cultivation methods for large-scale production.

Objective

To optimize culture conditions for large-scale production of CS1197 biomass and metabolites production, specifically focusing on maximizing biomass yield, adenosine and cordycepin content through submerged cultivation.

Results

The optimal culture conditions were determined to be temperature 28°C, pH 7, and inoculum volume 10% for maximum overall desirability of biomass, adenosine and cordycepin production. Under these conditions, the biomass yield was 4.53 g/L with adenosine and cordycepin yields showing only 20% and 31.5% reduction from their maximum possible yields respectively. The water extracts showed good antioxidant activity through DPPH and ABTS radical scavenging.

Conclusion

The study successfully optimized submerged cultivation conditions for CS1197 using response surface methodology and desirability function analysis. The presence of important biomarkers adenosine and cordycepin was confirmed in the cultivated biomass, with yields comparable to natural specimens. The optimized process conditions provide a viable approach for large-scale production of CS biomass and metabolites.
  • Published in:Pharmacognosy Magazine,
  • Study Type:Laboratory Optimization Study,
  • Source: 10.4103/0973-1296.168946
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