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Complete genome sequence of Diaporthe vaccinii Shear, a fungal isolated from blueberry

Summary

Scientists have sequenced the complete genetic code of a fungus that damages blueberry crops. This fungus, called Diaporthe vaccinii, causes diseases like twig blight and fruit rot that cost farmers significant money. The complete genome sequence provides valuable information for understanding how the fungus causes disease and may help develop better ways to protect blueberry crops.

Background

Diaporthe vaccinii is a destructive fungal pathogen of blueberry crops that causes significant economic losses through various disease manifestations including twig blight, stem canker, and postharvest fruit rot. Understanding the molecular virulence determinants of this pathogen requires high-quality genomic information.

Objective

To obtain a complete genome sequence of Diaporthe vaccinii strain CBS 118571 using hybrid sequencing approaches to elucidate its molecular virulence determinants and support future pathogenesis research.

Results

The complete genome assembly comprises 64 contigs with a total length of 60.2 Mb and GC content of 48.3%. BUSCO analysis confirmed 99.3% of conserved fungal orthologs as complete, with 97.99% of Illumina reads and 89.92% of ONT reads successfully mapping to the assembly, demonstrating exceptional assembly quality and continuity.

Conclusion

A high-quality complete genome sequence of Diaporthe vaccinii has been successfully generated using hybrid sequencing. This genomic resource will facilitate future studies of pathogenic mechanisms and support crop protection strategies for commercial blueberry production.
  • Published in:Microbiology Resource Announcements,
  • Study Type:Genome Sequencing Study,
  • Source: 10.1128/mra.00001-25, PMID: 40862361
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