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Complete genome sequence of Diaporthe vaccinii Shear, a fungal isolated from blueberry

Summary

Scientists sequenced the complete genetic blueprint of Diaporthe vaccinii, a harmful fungus that damages blueberry plants and costs farmers significant money. Using advanced DNA sequencing technology, they assembled a high-quality genetic map of the fungus to better understand how it causes disease. This genetic information could help researchers develop better ways to protect blueberry crops from this destructive pathogen.

Background

Diaporthe vaccinii Shear is a destructive plant pathogen causing substantial economic losses in commercial Vaccinium crops through necrotrophic pathogenesis manifesting as twig blight, stem canker, dieback, leaf chlorosis, and postharvest fruit rot. The strain CBS 118571 was isolated from blueberry in Michigan, USA and deposited in the Westerdijk Fungal Biodiversity Institute.

Objective

To elucidate the molecular virulence determinants of D. vaccinii by obtaining a high-contiguity de novo genome assembly using hybrid sequencing approaches.

Results

The hybrid genome assembly comprises 64 contigs with a total length of 60.2 Mb and GC content of 48.3%. BUSCO analysis identified 99.3% of conserved fungal orthologs as complete, with 97.99% of Illumina reads and 89.92% of ONT reads successfully mapping to the assembly.

Conclusion

The complete genome sequence of D. vaccinii CBS 118571 was successfully obtained with exceptional assembly continuity, providing a high-quality reference for investigating the molecular basis of its virulence and pathogenicity in blueberry crops.
  • Published in:Microbiology Resource Announcements,
  • Study Type:Genomic Analysis,
  • Source: 10.1128/mra.00001-25, PMID: 40862361
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