Comparison of Polysaccharides Extracted from Cultivated Mycelium of Inonotus obliquus with Polysaccharide Fractions Obtained from Sterile Conk (Chaga) and Birch Heart Rot

Summary

This research compared the chemical structures of beneficial compounds called polysaccharides extracted from different sources of the medicinal mushroom Chaga. The study found important differences between polysaccharides from lab-grown mushroom cells versus wild-harvested Chaga, which could affect their health benefits. Key impacts on everyday life include: • Provides scientific basis for developing sustainable lab-grown alternatives to wild-harvested Chaga products • Helps explain why traditional Chaga preparations may have different effects than newer cultivated products • Suggests new ways to utilize waste wood from Chaga harvesting for beneficial compounds • Contributes to more efficient production of natural health supplements • Advances understanding of how to verify quality and potency of Chaga products

Background

Inonotus obliquus is a circumboreal basidiomycete that grows as a parasite on birch trees, producing a black sterile conk known as Chaga. The polysaccharides extracted from Chaga have demonstrated multiple bioactivities including antioxidant, anti-tumor and immunomodulatory effects. Due to sustainability concerns with wild harvesting, cultivated mycelium has been proposed as an alternative source of these bioactive compounds. However, limited research has compared the structural differences between polysaccharides from different sources.

Objective

To extract and characterize polysaccharides from cultivated I. obliquus mycelium and compare their properties with polysaccharides extracted from wild Chaga sterile conks and infected birch heart rot. The study aimed to assess structural differences that could impact bioactivity and nutraceutical potential.

Results

High amounts of phenolic compounds were found bound to sterile conk polysaccharides but not in mycelial extracts. Mycelial polysaccharides were rich in α- and β-glucans with both high (105 Da) and low (104 Da) molecular weight populations. Sterile conk polysaccharides contained mainly β-glucan of lower molecular weight (103 Da). Heart rot polysaccharides were primarily low molecular weight (103 Da) hemicelluloses with some fungal polysaccharides present.

Conclusion

The study revealed significant differences in composition, structure and molecular weight between polysaccharides from cultivated mycelium versus sterile conk and heart rot. Mycelial extracts contained more glycogen and showed polydispersed molecular weights, while conk extracts had phenolic compounds bound to lower weight β-glucans. These structural differences likely impact their biological activities and nutraceutical potential. The presence of bioactive polysaccharides in heart rot suggests potential for utilizing this waste stream.
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