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Comparative Transcriptome Analysis Identified Candidate Genes Involved in Mycelium Browning in Lentinula edodes

Summary

This research investigated how shiitake mushrooms develop their characteristic brown surface coating, which is essential for healthy mushroom growth and protection. Scientists studied the genetic mechanisms that control this browning process by comparing normal and abnormal brown surface formation. Impacts on everyday life: – Helps improve commercial mushroom cultivation techniques – Could lead to better quality and higher yields of shiitake mushrooms – Provides insights for developing more resistant mushroom strains – May reduce crop losses from contamination and disease – Could make mushroom farming more efficient and cost-effective

Background

Lentinula edodes is one of the most popular edible mushroom species globally, contributing approximately 22% of world mushroom supply. It contains useful medicinal components like lentinan. The light-induced formation of brown film on vegetative mycelial tissues is a critical process for ensuring quantity and quality of this edible mushroom.

Objective

To understand the molecular mechanisms underlying the critical developmental process of brown film formation in L. edodes by characterizing morphological phenotypic changes in the Chamaram strain associated with abnormal brown film formation and comparing its genome-wide transcriptional features.

Results

The analysis identified clusters of genes specific to light-induced brown film phenotypes. These genes were significantly associated with light sensing via photoreceptors like FMN- and FAD-bindings, signal transduction by kinases and GPCRs, melanogenesis via activation of tyrosinases, and cell wall degradation by glucanases, chitinases, and laccases. Hydrophobin genes SC1 and SC3 showed divergent expression levels in normal versus abnormal brown mycelial films. Glycoside hydrolase domain-containing genes were up-regulated in normal brown film but not in abnormal film phenotype.

Conclusion

The study systematically analyzed expression patterns of light-induced browning-related genes in L. edodes, revealing key molecular mechanisms involved in brown film formation. The findings provide valuable information for investigating browning formation mechanisms and establish a foundation for future L. edodes breeding programs.
  • Published in:BMC Genomics,
  • Study Type:Comparative Transcriptome Analysis,
  • Source: 10.1186/s12864-019-5509-4
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