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Comparative Transcriptome Analysis Identified Candidate Genes Involved in Mycelium Browning in Lentinula edodes

Summary

This research investigated how shiitake mushrooms develop their characteristic brown surface coating, which is essential for healthy mushroom growth and production. Scientists studied the genes that are activated when mushrooms are exposed to light, leading to this brown coating formation. Understanding this process is crucial for mushroom farmers and could lead to better cultivation methods. Impacts on everyday life: – Helps improve commercial mushroom cultivation techniques – Could lead to higher quality and quantity of edible mushrooms in markets – Provides insights for developing more resistant mushroom strains – May reduce mushroom crop losses due to improper brown film formation – Could result in more cost-effective mushroom production methods

Background

Lentinula edodes is one of the most popular edible mushroom species globally, contributing about 22% of world mushroom supply. It contains useful medicinal components like lentinan. The light-induced formation of brown film on vegetative mycelial tissues is a critical process for ensuring quantity and quality of this edible mushroom. Understanding the molecular mechanisms underlying this developmental process is important for mushroom cultivation.

Objective

To understand the molecular mechanisms of brown film formation in L. edodes by characterizing morphological phenotypic changes in the Chamaram strain associated with abnormal brown film formation and comparing its genome-wide transcriptional features.

Results

The analysis identified clusters of genes specific to light-induced brown film phenotypes. These genes were significantly associated with light sensing via photoreceptors like FMN- and FAD-bindings, signal transduction by kinases and GPCRs, melanogenesis via activation of tyrosinases, and cell wall degradation by glucanases, chitinases, and laccases. Hydrophobin genes SC1 and SC3 showed divergent expression levels in normal versus abnormal brown films. Glycoside hydrolase domain-containing genes were up-regulated in normal brown film but not in abnormal phenotype.

Conclusion

The study systematically analyzed expression patterns of light-induced browning-related genes in L. edodes, providing information for investigating browning formation mechanisms and establishing a foundation for future L. edodes breeding. The findings revealed key molecular pathways involved in normal versus abnormal brown film formation.
  • Published in:BMC Genomics,
  • Study Type:Comparative Transcriptome Analysis,
  • Source: 10.1186/s12864-019-5509-4
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