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Comparative proteomics reveals the mechanism of cyclosporine production and mycelial growth in Tolypocladium inflatum affected by different carbon sources

Summary

Scientists studied how different sugar sources (fructose versus sucrose) affect the production of cyclosporine A, an important drug used to prevent organ rejection after transplants. Using advanced protein analysis techniques, they identified which proteins were more active in each sugar environment and discovered that fructose promotes drug production while sucrose promotes fungal growth. This research could help pharmaceutical companies produce cyclosporine more efficiently by identifying key proteins to enhance.

Background

Cyclosporine A (CsA) is an important immunosuppressant produced by the fungus Tolypocladium inflatum. CsA production and mycelial growth vary depending on culture conditions, particularly carbon sources. Understanding the molecular mechanisms underlying these differences could improve industrial production efficiency.

Objective

This study aimed to identify proteins and metabolic pathways that regulate CsA production and mycelial growth in T. inflatum cultured in different carbon source media using comparative proteomics analysis.

Results

Fructose medium favored CsA production while sucrose medium promoted mycelial growth. Analysis identified 244 DEPs (74 upregulated in fructose, 170 in sucrose) involved in carbon metabolism, lipid metabolism, amino acid biosynthesis, stress response, and cell wall integrity. Key hub proteins regulating CsA synthesis and mycelial growth were identified through network analysis.

Conclusion

This comparative proteomics study reveals the molecular mechanisms by which different carbon sources differentially regulate CsA production and mycelial growth in T. inflatum, providing candidate genes and proteins for metabolic engineering strategies to develop higher-yielding strains.
  • Published in:Frontiers in Microbiology,
  • Study Type:Experimental Study,
  • Source: PMC10757567, PMID: 38163081
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