Comparative Proteomic Analysis Reveals Differential Protein Expression of Hypsizygus marmoreus in Response to Different Light Qualities

Summary

This research examined how different colors of light affect protein production in an edible mushroom species. Understanding these effects helps optimize mushroom cultivation conditions. Impact on everyday life: – Improved commercial mushroom production methods – Better quality control in mushroom farming – More efficient growing conditions for edible mushrooms – Potential cost savings in mushroom cultivation – Enhanced nutritional value through optimized growing conditions

Background

Hypsizygus marmoreus is a commercially valuable mushroom cultivated in Asia known for its organoleptic and medicinal properties. Light is a key environmental factor affecting mushroom growth, development and metabolism, but the molecular basis of light effects on H. marmoreus remains unclear.

Objective

To investigate the global protein expression profile of H. marmoreus mycelia growing under white, red, green, and blue light qualities compared to darkness using label-free comparative proteomic analysis.

Results

Among 3149 identified proteins, 2288 were expressed across all tested conditions. Numerous differentially expressed proteins were identified between dark and light conditions, with white light showing the most changes. KEGG analysis revealed light stress was associated with primary metabolism, glycolysis/gluconeogenesis, MAPK, proteasome, and carbohydrate-active enzyme pathways.

Conclusion

The study provided valuable insights into molecular mechanisms underlying how different light qualities affect mushroom growth and development through proteomic analysis. Key pathways and protein changes were identified that help explain H. marmoreus response to various light conditions.
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