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Comparative Proteome Reveals Metabolic Changes During the Fruiting Process in Flammulina velutipes

Summary

This research examined how proteins change when mushrooms develop from simple thread-like structures into full mushroom bodies. The study focused on the golden needle mushroom (Flammulina velutipes), an economically important edible mushroom species. The findings help explain how mushrooms develop and could lead to better cultivation methods. Impacts on everyday life: • Could lead to more efficient mushroom farming techniques • May help improve the quality of commercially grown mushrooms • Contributes to understanding how to optimize mushroom growth conditions • Could help reduce costs in commercial mushroom production • May lead to better yields for mushroom farmers

Background

Understanding the molecular mechanisms regulating the fruiting process in macro-fungi, especially industrially cultivated mushrooms, has been a long-standing goal in mycological research. The transformation of mycelia into fruit-bodies in Flammulina velutipes involves complex metabolic changes that need to be better understood.

Objective

To investigate proteins expressed characteristically and abundantly at primordium and fruit-body stages in Flammulina velutipes using iTRAQ labeling technique, in order to gain insights into the events accompanying mushroom fruiting body development.

Results

The study identified 171 differentially expressed proteins, with 68 showing up-regulated expression levels associated with 84 specific KEGG pathways. Key up-regulated proteins included pyruvate carboxylase, aldehyde dehydrogenase, fatty acid synthase, aspartate aminotransferase, 2-cysteine peroxiredoxin, FDS protein, translation elongation factor 1-alpha, mitogen-activated protein kinases (MAPKs), and heat-shock protein 70.

Conclusion

The identified proteins could serve as potential stage-specific biomarkers for studying the fruiting process in F. velutipes. These findings provide valuable evidence for understanding the molecular mechanisms of fruit-body initiation and development in basidiomycete fungi, while also offering practical insights for yield improvement and quality control of super golden-needle mushroom production.
  • Published in:Journal of Agricultural and Food Chemistry,
  • Study Type:Comparative Study,
  • Source: 10.1021/acs.jafc.7b01120
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