Comparative genome analysis of patulin-producing Penicillium paneum OM1 isolated from pears

Summary

Scientists sequenced the genome of a pear fungus called Penicillium paneum that produces patulin, a toxic compound found in moldy apples and pears. By analyzing its genetic blueprint, researchers identified 33 different toxin-producing gene clusters, with special focus on the 15 genes responsible for patulin production. The findings reveal which genes P. paneum uses to make patulin and how they compare to other fungal species, potentially helping develop better ways to prevent patulin contamination in fruit and fruit products.

Background

Penicillium paneum produces patulin, a toxic secondary metabolite found on apples and pears. Little is known about the biosynthetic gene clusters of secondary metabolites, including patulin, in P. paneum. This study aimed to sequence and analyze the genome of a patulin-producing P. paneum strain isolated from pears.

Objective

To sequence the whole genome of P. paneum isolate OM1 and identify its secondary metabolite biosynthetic gene clusters, particularly the patulin BGC, and compare it with patulin-producing strains. Additionally, to investigate carbohydrate-active enzymes and analyze phylogenetic relationships with related Penicillium species.

Results

The P. paneum OM1 genome is approximately 27.16 Mb with four chromosomes and 10,679 protein-coding genes. A total of 33 secondary metabolite BGCs were identified, including a complete patulin BGC with all 15 genes. The patulin biosynthetic genes showed high sequence identity (>73%) with other patulin-producing Penicillium strains but low identity with non-patulin producers.

Conclusion

This study improves understanding of secondary metabolite biosynthetic gene clusters in P. paneum OM1 and provides genetic basis for patulin biosynthesis. The findings could support development of strategies to reduce patulin contamination on pome fruits such as apples and pears.
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