Comparative Analysis of Simulated In-Situ Colonization and Degradation by Lentinula edodes on Oak Wafer and Corn Stalk
- Author: mycolabadmin
- 2023-11-23
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Summary
This research examined how shiitake mushroom fungi break down wood and plant materials differently. The study provides insights into how these fungi can be used to convert plant waste into useful products. Key impacts on everyday life include:
• More efficient production of shiitake mushrooms for food
• Better methods for recycling agricultural waste materials
• Development of eco-friendly processes for breaking down plant materials
• Improved understanding of natural decomposition processes
• Potential applications in biofuel and biomaterial production
Background
The depolymerization of lignocellulose biomass by white-rot fungi is an important research topic, but few simulated in-situ analyses have been conducted to understand the decay process. Lentinula edodes (shiitake mushroom) is a white-rot fungus capable of efficiently degrading cellulose, hemicellulose and lignin through secreted enzymes.
Objective
To analyze and compare the hyphal transcriptional response and substrate degradation during spatial-temporal colonization of L. edodes on different substrates (oak wafer and corn stalk) to better understand the mechanisms of lignocellulose depolymerization.
Results
Faster growth and thicker hyphal mat was observed on corn stalk compared to oak wafer. Higher lignin oxidase activity was detected on oak wafer, while higher cellulase activity was found on corn stalk which contained more soluble sugars. Numerous carbohydrate-binding module containing enzyme genes were significantly upregulated in back-distal hyphae on corn stalk. The hyphae preferentially colonized and degraded the secondary cell wall. Lignin was degraded as a priority in oak wafer, with the three lignin monomers being degraded simultaneously in oak wafer but sequentially in corn stalk.
Conclusion
The white-rot degradation pattern of lignocellulose is determined by the chemical composition and structure of the colonized biomass. L. edodes employs different degradation strategies based on substrate type, with higher oxidative enzyme activity on oak wafer and increased hydrolytic enzyme activity on corn stalk. The research provides important guidance about plant cell wall degradation characteristics of L. edodes in early colonization phases of wood and straw for effective biomass conversion.
- Published in:Frontiers in Microbiology,
- Study Type:Laboratory Research Study,
- Source: 10.3389/fmicb.2023.1286064